C1992

ColorBurst (Cat. No. C1992): Bands transferred to nitrocellulose membranes. Transfers were completed in 90 minutes at 70 volts with Towbin’s buffer (Tris-Glycine in 20% methanol). Molecular Masses (Da) given are apparent values as compared to Sigma’s wide range marker set (Cat. No. S8445). The ColorBurst markers migrate differently with respect to Cat. No. S8445 in the different gel systems tested.

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C1992

Sigma

 

ColorBurst Electrophoresis Marker

mol wt 8,000-220,000 Da

MDL number:MFCD03456450

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Description

ComponentsSolution contains myosin-violet, BSA-red, GDH-blue, ADH-red, Carbonic Anhydrase-orange, Trypsin Inhibitor-blue, Lysozyme-red, Aprotinin-blue, 200 mM Tris, SDS and, formamide. Each lot provides the apparent molecular weights determined by comparison to an unconjugated standard.
ApplicationColorburst can be used to estimate sample molecular weights, to monitor the progress of an electrophoretic run, or to confirm that an electroblot is complete.
 Brilliantly colored, exceptionally well-resolved, convenient, and stable, ColorBurst protein molecular weight markers perform impressively in a variety of gel compositions and concentrations. ColorBurst Markers are composed of 8 polypeptides which have been chemically reduced and conjugated to brilliantly colored dyes. Conveniently, ColorBurst Markers require no resuspension, reduction, or heating prior to use.
QuantityEach vial of ColorBurst contains 500 μL of solution, enough for at least 50 mini gel applications.
Legal InformationColorBurst is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

formsolution
mol wtmol wt 8,000-220,000 Da
pH8
storage temp.−20°C

Safety

Hazard CodesT
Risk Statements61
Safety Statements53-45
WGK Germany1